ceacam1 mouse igg2b n a pe r d systems  (R&D Systems)

Journal: bioRxiv

Article Title: Dynamics of the CD9 interactome during bacterial infection of epithelial cells by proximity labelling proteomics

doi: 10.1101/2024.12.13.628358

Figure Lengend Snippet: (A) CD9 knockout disrupts both meningococcal and staphylococcal adherence. WT and CD9 -/- cells were infected with either meningococci (MC58) or staphylococci (SH1000) for 60 mins at an MOI=50. Cells were disrupted after infection and adherent and internalised bacteria were enumerated by colony forming units (cfu). (B-C) CD9-derived peptide, 800C, reduces meningococcal adherence (B) and staphylcoccal adherence (C) to epithelial cells. WT or CD9 -/- cells were pre-treated with a scrambled peptide or 800C for 60 mins prior to infection. Cells were infected as above and adherence enumerated by cfu. (D) Cell surface expression of meningococcal receptors measured by flow cytometry. WT or CD9 -/- cells were treated with an anti-CD9 antibody (602.29), an anti-CD46 antibody, an anti-CD147 antibody. A mouse IgG isotype control (JC1) was included and expression was determined using a FITC-conjugated secondary antibody. % change was calculated by comparing WT to CD9 -/- cells. (E) Representative blot demonstrating expression of meningococcal and staphylococcal receptors. Whole cell lysates of WT and CD9 -/- cells were electrophoresed and blotted. Blots were probed with an anti-CD44 antibody. An anti-GAPDH antibody was used as a loading control. Densitometry was calculated through ImageJ analysis, removing background with an empty lane and normalising to the loading control. n > 3, mean + SEM.

Article Snippet: Mouse anti-human CD9 IgG1 (MM2/57; Merck, Germany), mouse anti-human CD9 IgG1 (602.29; kind gift of Lynda Partridge, University of Sheffield, Sheffield, UK), mouse anti-human CD147 IgG1 (HIM6; Biolegend, California, USA), mouse anti-human CD46 IgG1 (MEM-258; Thermo Fisher Scientific), mouse anti-human CEACAM1 IgG1 (B3-17; Merck), mouse anti-human CEACAM6 IgG1 (1H7-4B; Merck), mouse anti-human CD44 IgG2a (60224-1-Ig; Proteintech Group, Inc, Illinois, USA), mouse anti-human GAPDH (MAB374; Merck), mouse IgG1 (JC1; in house), mouse IgG2a (02-6200; Thermofisher Scientific), mouse anti-FLAG (M2; Merck), goat anti-mouse HRP (P0447; Agilent, California, USA) were used as described.

Techniques: Knock-Out, Infection, Bacteria, Derivative Assay, Expressing, Flow Cytometry, Control

Journal: Discover. Oncology

Article Title: Radiotherapy combined with anti-CEACAM1 immunotherapy to induce survival advantage in glioma

doi: 10.1007/s12672-023-00638-x

Figure Lengend Snippet: Expression of CEACAM1 on T-lymphocytes in the peripheral blood of postoperative patients with glioma before and after radiotherapy. A , B Flow-cytometric graphs of the expression of CEACAM1 on CD4 + and CD8 + T-lymphocytes in the peripheral blood of patients with postoperative glioma before and after radiotherapy. C Statistical graph of the percentage of CEACAM1 + CD4 + T-cells. D Statistical graph of the percentage of CEACAM1 + CD8 + T-cells. *: P < 0.05, **: P < 0.01

Article Snippet: Antibodies for flow cytometry and immunohistochemistry were as follows: anti-mouse CD3e (Clone 145-2C11; Cat. #: 15–0031-82; eBioscience, San Diego, CA, USA), anti-mouse CD4 (Clone GK1.5; Cat. #: 12–0041-82; eBioscience), anti-mouse CD8a (Clone 53–6.7; Cat. #: 11–0081-82; eBioscience), anti-mouse CD25 (Clone PC61.5; Cat. #: 25–0251-82; eBioscience), anti-mouse Foxp3 (Clone FJK-16 s; Cat. #: 11–5773-82; eBioscience), anti-mouse CEACAM1 (Clone 723629; Cat. #: MA5-24338; eBioscience), anti- human CD3 (Clone OKT3; Cat. #: 14–0037-82; eBioscience), anti- human CD4 (Clone RPA-T4; Cat. #: 11–0049-42; eBioscience), anti-mouse CD8a (Clone RPA-T8; Cat. #: 12–0088-42; eBioscience), anti-human CEACAM1 (Clone YTH71.3; Cat. #: MA5-17003; eBioscience),anti-mouse ki-67(SAB5700770; Sigma-Aldrich) and In Situ Cell Death Detection Kit (11684817910;Roche).

Techniques: Expressing

Journal: Discover. Oncology

Article Title: Radiotherapy combined with anti-CEACAM1 immunotherapy to induce survival advantage in glioma

doi: 10.1007/s12672-023-00638-x

Figure Lengend Snippet: Expression of CEACAM1 in murine intracranial glioma cells before radiotherapy, 1 week and 1 month after radiotherapy as detected by immunohistochemistry. A–C Representative images of immunohistochemically stained murine glioma tissues at different time windows before and after radiotherapy. D Immunohistochemistry staining intensity scores. **: P < 0.01

Article Snippet: Antibodies for flow cytometry and immunohistochemistry were as follows: anti-mouse CD3e (Clone 145-2C11; Cat. #: 15–0031-82; eBioscience, San Diego, CA, USA), anti-mouse CD4 (Clone GK1.5; Cat. #: 12–0041-82; eBioscience), anti-mouse CD8a (Clone 53–6.7; Cat. #: 11–0081-82; eBioscience), anti-mouse CD25 (Clone PC61.5; Cat. #: 25–0251-82; eBioscience), anti-mouse Foxp3 (Clone FJK-16 s; Cat. #: 11–5773-82; eBioscience), anti-mouse CEACAM1 (Clone 723629; Cat. #: MA5-24338; eBioscience), anti- human CD3 (Clone OKT3; Cat. #: 14–0037-82; eBioscience), anti- human CD4 (Clone RPA-T4; Cat. #: 11–0049-42; eBioscience), anti-mouse CD8a (Clone RPA-T8; Cat. #: 12–0088-42; eBioscience), anti-human CEACAM1 (Clone YTH71.3; Cat. #: MA5-17003; eBioscience),anti-mouse ki-67(SAB5700770; Sigma-Aldrich) and In Situ Cell Death Detection Kit (11684817910;Roche).

Techniques: Expressing, Immunohistochemistry, Staining

Journal: Discover. Oncology

Article Title: Radiotherapy combined with anti-CEACAM1 immunotherapy to induce survival advantage in glioma

doi: 10.1007/s12672-023-00638-x

Figure Lengend Snippet: Survival prediction of CEACAM1/Radio in glioma. A , B Kaplan–Meier analysis of CEACAM1 expression in the Chinese Glioma Genome Atlas (CGGA) and The Cancer Genome Atlas (TCGA) databases. The cutoff of the group is the median expression of CEACAM1.The significance of the prognostic value was tested by a log-rank test. C Kaplan­Meier analysis of Radio in the Chinese Glioma Genome Atlas (CGGA) database

Article Snippet: Antibodies for flow cytometry and immunohistochemistry were as follows: anti-mouse CD3e (Clone 145-2C11; Cat. #: 15–0031-82; eBioscience, San Diego, CA, USA), anti-mouse CD4 (Clone GK1.5; Cat. #: 12–0041-82; eBioscience), anti-mouse CD8a (Clone 53–6.7; Cat. #: 11–0081-82; eBioscience), anti-mouse CD25 (Clone PC61.5; Cat. #: 25–0251-82; eBioscience), anti-mouse Foxp3 (Clone FJK-16 s; Cat. #: 11–5773-82; eBioscience), anti-mouse CEACAM1 (Clone 723629; Cat. #: MA5-24338; eBioscience), anti- human CD3 (Clone OKT3; Cat. #: 14–0037-82; eBioscience), anti- human CD4 (Clone RPA-T4; Cat. #: 11–0049-42; eBioscience), anti-mouse CD8a (Clone RPA-T8; Cat. #: 12–0088-42; eBioscience), anti-human CEACAM1 (Clone YTH71.3; Cat. #: MA5-17003; eBioscience),anti-mouse ki-67(SAB5700770; Sigma-Aldrich) and In Situ Cell Death Detection Kit (11684817910;Roche).

Techniques: Expressing

Journal: Discover. Oncology

Article Title: Radiotherapy combined with anti-CEACAM1 immunotherapy to induce survival advantage in glioma

doi: 10.1007/s12672-023-00638-x

Figure Lengend Snippet: Univariate and multivariate analysis of prognostic parameters in the Chinese Glioma Genome Atlas (CGGA) database overall survival (OS)

Article Snippet: Antibodies for flow cytometry and immunohistochemistry were as follows: anti-mouse CD3e (Clone 145-2C11; Cat. #: 15–0031-82; eBioscience, San Diego, CA, USA), anti-mouse CD4 (Clone GK1.5; Cat. #: 12–0041-82; eBioscience), anti-mouse CD8a (Clone 53–6.7; Cat. #: 11–0081-82; eBioscience), anti-mouse CD25 (Clone PC61.5; Cat. #: 25–0251-82; eBioscience), anti-mouse Foxp3 (Clone FJK-16 s; Cat. #: 11–5773-82; eBioscience), anti-mouse CEACAM1 (Clone 723629; Cat. #: MA5-24338; eBioscience), anti- human CD3 (Clone OKT3; Cat. #: 14–0037-82; eBioscience), anti- human CD4 (Clone RPA-T4; Cat. #: 11–0049-42; eBioscience), anti-mouse CD8a (Clone RPA-T8; Cat. #: 12–0088-42; eBioscience), anti-human CEACAM1 (Clone YTH71.3; Cat. #: MA5-17003; eBioscience),anti-mouse ki-67(SAB5700770; Sigma-Aldrich) and In Situ Cell Death Detection Kit (11684817910;Roche).

Techniques:

Journal: Discover. Oncology

Article Title: Radiotherapy combined with anti-CEACAM1 immunotherapy to induce survival advantage in glioma

doi: 10.1007/s12672-023-00638-x

Figure Lengend Snippet: Univariate and multivariate analysis of prognostic parameters in The Cancer Genome Atlas (TCGA) database overall survival (OS)

Article Snippet: Antibodies for flow cytometry and immunohistochemistry were as follows: anti-mouse CD3e (Clone 145-2C11; Cat. #: 15–0031-82; eBioscience, San Diego, CA, USA), anti-mouse CD4 (Clone GK1.5; Cat. #: 12–0041-82; eBioscience), anti-mouse CD8a (Clone 53–6.7; Cat. #: 11–0081-82; eBioscience), anti-mouse CD25 (Clone PC61.5; Cat. #: 25–0251-82; eBioscience), anti-mouse Foxp3 (Clone FJK-16 s; Cat. #: 11–5773-82; eBioscience), anti-mouse CEACAM1 (Clone 723629; Cat. #: MA5-24338; eBioscience), anti- human CD3 (Clone OKT3; Cat. #: 14–0037-82; eBioscience), anti- human CD4 (Clone RPA-T4; Cat. #: 11–0049-42; eBioscience), anti-mouse CD8a (Clone RPA-T8; Cat. #: 12–0088-42; eBioscience), anti-human CEACAM1 (Clone YTH71.3; Cat. #: MA5-17003; eBioscience),anti-mouse ki-67(SAB5700770; Sigma-Aldrich) and In Situ Cell Death Detection Kit (11684817910;Roche).

Techniques:

Journal: Discover. Oncology

Article Title: Radiotherapy combined with anti-CEACAM1 immunotherapy to induce survival advantage in glioma

doi: 10.1007/s12672-023-00638-x

Figure Lengend Snippet: Association between CEACAM1 and clinicopathological characteristics of gliomas. A The landscape of CEACAM1-related clinicopathological features of gliomas in the Chinese Glioma Genome Atlas (CGGA) database. B The landscape of CEACAM1-­relatedclinicopathological features of gliomas in the The Cancer Genome Atlas (TCGA) database. C – F Distribution of glioma-related gene CEACAM1 in MGMT, IDH, Grade and 1p19q. G–J Distribution of glioma-related gene CEACAM1 in MGMT, IDH, Grade and 1p19q

Article Snippet: Antibodies for flow cytometry and immunohistochemistry were as follows: anti-mouse CD3e (Clone 145-2C11; Cat. #: 15–0031-82; eBioscience, San Diego, CA, USA), anti-mouse CD4 (Clone GK1.5; Cat. #: 12–0041-82; eBioscience), anti-mouse CD8a (Clone 53–6.7; Cat. #: 11–0081-82; eBioscience), anti-mouse CD25 (Clone PC61.5; Cat. #: 25–0251-82; eBioscience), anti-mouse Foxp3 (Clone FJK-16 s; Cat. #: 11–5773-82; eBioscience), anti-mouse CEACAM1 (Clone 723629; Cat. #: MA5-24338; eBioscience), anti- human CD3 (Clone OKT3; Cat. #: 14–0037-82; eBioscience), anti- human CD4 (Clone RPA-T4; Cat. #: 11–0049-42; eBioscience), anti-mouse CD8a (Clone RPA-T8; Cat. #: 12–0088-42; eBioscience), anti-human CEACAM1 (Clone YTH71.3; Cat. #: MA5-17003; eBioscience),anti-mouse ki-67(SAB5700770; Sigma-Aldrich) and In Situ Cell Death Detection Kit (11684817910;Roche).

Techniques:

Journal: Discover. Oncology

Article Title: Radiotherapy combined with anti-CEACAM1 immunotherapy to induce survival advantage in glioma

doi: 10.1007/s12672-023-00638-x

Figure Lengend Snippet: CEACAM1 is closely associated with inflammatory response regulation in gliomas. A – C Biological processes (BP), cellular components (CC), and molecular functions (MF) are mostly related to CEACAM1 in the Chinese Glioma Genome Atlas (CGGA) database. D – F Biological processes (BP), cellular components (CC), and molecular functions (MF) are mostly related to CEACAM1 in the TCGA database. G Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of CCEACAM1 in the CGGA database. H KEGG pathway analysis of CEACAM1 in the TCGA database

Article Snippet: Antibodies for flow cytometry and immunohistochemistry were as follows: anti-mouse CD3e (Clone 145-2C11; Cat. #: 15–0031-82; eBioscience, San Diego, CA, USA), anti-mouse CD4 (Clone GK1.5; Cat. #: 12–0041-82; eBioscience), anti-mouse CD8a (Clone 53–6.7; Cat. #: 11–0081-82; eBioscience), anti-mouse CD25 (Clone PC61.5; Cat. #: 25–0251-82; eBioscience), anti-mouse Foxp3 (Clone FJK-16 s; Cat. #: 11–5773-82; eBioscience), anti-mouse CEACAM1 (Clone 723629; Cat. #: MA5-24338; eBioscience), anti- human CD3 (Clone OKT3; Cat. #: 14–0037-82; eBioscience), anti- human CD4 (Clone RPA-T4; Cat. #: 11–0049-42; eBioscience), anti-mouse CD8a (Clone RPA-T8; Cat. #: 12–0088-42; eBioscience), anti-human CEACAM1 (Clone YTH71.3; Cat. #: MA5-17003; eBioscience),anti-mouse ki-67(SAB5700770; Sigma-Aldrich) and In Situ Cell Death Detection Kit (11684817910;Roche).

Techniques:

Journal: PLoS ONE

Article Title: Neutrophils Infiltration in the Tongue Squamous Cell Carcinoma and Its Correlation with CEACAM1 Expression on Tumor Cells

doi: 10.1371/journal.pone.0089991

Figure Lengend Snippet: The expression of CEACAM1 on different TSCC groups and its relationship with clinicopathologic features.

Article Snippet: The membrane was blocked with 5% non-fat milk for 1 h, and incubated with monoclonal mouse anti-human CEACAM1 (dilution 1∶500, RD; MAB2244, USA) antibodies for 2 h at room temperature, followed by washing with TBST (TBS containing 0.1% Tween 20) for 5 min five times.

Techniques: Expressing

Journal: PLoS ONE

Article Title: Neutrophils Infiltration in the Tongue Squamous Cell Carcinoma and Its Correlation with CEACAM1 Expression on Tumor Cells

doi: 10.1371/journal.pone.0089991

Figure Lengend Snippet: The relationship of CEACAM1 expression and density of neutrophils.

Article Snippet: The membrane was blocked with 5% non-fat milk for 1 h, and incubated with monoclonal mouse anti-human CEACAM1 (dilution 1∶500, RD; MAB2244, USA) antibodies for 2 h at room temperature, followed by washing with TBST (TBS containing 0.1% Tween 20) for 5 min five times.

Techniques: Expressing

Journal: PLoS ONE

Article Title: Neutrophils Infiltration in the Tongue Squamous Cell Carcinoma and Its Correlation with CEACAM1 Expression on Tumor Cells

doi: 10.1371/journal.pone.0089991

Figure Lengend Snippet: Univariate and Multivariate survival analysis in TSCC patients.

Article Snippet: The membrane was blocked with 5% non-fat milk for 1 h, and incubated with monoclonal mouse anti-human CEACAM1 (dilution 1∶500, RD; MAB2244, USA) antibodies for 2 h at room temperature, followed by washing with TBST (TBS containing 0.1% Tween 20) for 5 min five times.

Techniques: Expressing

Journal: PLoS ONE

Article Title: Neutrophils Infiltration in the Tongue Squamous Cell Carcinoma and Its Correlation with CEACAM1 Expression on Tumor Cells

doi: 10.1371/journal.pone.0089991

Figure Lengend Snippet: Primer sequences and size of PCR products.

Article Snippet: The membrane was blocked with 5% non-fat milk for 1 h, and incubated with monoclonal mouse anti-human CEACAM1 (dilution 1∶500, RD; MAB2244, USA) antibodies for 2 h at room temperature, followed by washing with TBST (TBS containing 0.1% Tween 20) for 5 min five times.

Techniques:

Journal: PLoS ONE

Article Title: Neutrophils Infiltration in the Tongue Squamous Cell Carcinoma and Its Correlation with CEACAM1 Expression on Tumor Cells

doi: 10.1371/journal.pone.0089991

Figure Lengend Snippet: A: There were more CD15+ neutrophils (black arrow) in TSCC tissues (b, c, d) than in peritumoral tissues (a). In tumors areas, some neutrophils lied in the stroma of tumors (b), some were located within the carcinoma nests (c), and some infiltrated in the borderline of tumor invasion (d). (a: 100×; b, c, d: 400×). B: The expression of CEACAM1 in peritumoral tissues was negative or weak (a). In TSCC tissues, CEACAM1 expression was obviously stronger than that in peritumoral tissues and located mainly in the cytoplasm of tumor cells (b). (a: 100×; b: 400×). C: The relationship of CEACAM1 expression on tumor cells and infiltration of neutrophils. In strong CEACAM1 expression tumors, there were more neutrophils (black arrow) (a); While in negative or weak CEACAM1 expression tumors, there were relatively fewer neutrophils (b). (a, b: 200×, for CEACAM1).

Article Snippet: The membrane was blocked with 5% non-fat milk for 1 h, and incubated with monoclonal mouse anti-human CEACAM1 (dilution 1∶500, RD; MAB2244, USA) antibodies for 2 h at room temperature, followed by washing with TBST (TBS containing 0.1% Tween 20) for 5 min five times.

Techniques: Expressing

Journal: PLoS ONE

Article Title: Neutrophils Infiltration in the Tongue Squamous Cell Carcinoma and Its Correlation with CEACAM1 Expression on Tumor Cells

doi: 10.1371/journal.pone.0089991

Figure Lengend Snippet: A: The relationship between cumulative cancer-related survival of patients with resectable TSCC and density of neutrophils in tumor tissues. Patients with high density of neutrophils in tumors had significantly reduced cancer-related survival compared to low neutrophils density group ( P = 0.020, Log-rank test). B: The relationship between cumulative cancer-related survival of patients with resectable TSCC and CEACAM1 expression on tumor cells. Patients with moderate or strong expression of CEACAM1 had significantly reduced cancer-related survival compared to negative or weak CEACAM1 expression group ( P = 0.032, Log-rank test).

Article Snippet: The membrane was blocked with 5% non-fat milk for 1 h, and incubated with monoclonal mouse anti-human CEACAM1 (dilution 1∶500, RD; MAB2244, USA) antibodies for 2 h at room temperature, followed by washing with TBST (TBS containing 0.1% Tween 20) for 5 min five times.

Techniques: Expressing

Journal: PLoS ONE

Article Title: Neutrophils Infiltration in the Tongue Squamous Cell Carcinoma and Its Correlation with CEACAM1 Expression on Tumor Cells

doi: 10.1371/journal.pone.0089991

Figure Lengend Snippet: A: Fluorescence microscopic observation of Lv transfection efficientcy. a: CC1-4L-Lv group; b: CC1-4S-Lv group; c: Vector-Lv group. (a, b, c 200×). B: qRT- PCR results of Lv transfection. The results showed that CEACAM1-4L mRNA expression was obviously higher in CC1-4L-Lv transfection group than the other three groups (a), and CEACAM1-4S mRNA expression was the same as 4L (b). C: Western blot results for Lv transfection. a: β-actin; b: CEACAM1-4L (the above band) and -4S (the lower band). Results showed that CEACAM1-4L protein expression was obviously stronger in CC1-4L-Lv transfection group than the other three groups. CEACAM1-4S protein expression was the same as 4L. (Note: CEACAM1-4L protein was slightly bigger than CEACAM1-4S).

Article Snippet: The membrane was blocked with 5% non-fat milk for 1 h, and incubated with monoclonal mouse anti-human CEACAM1 (dilution 1∶500, RD; MAB2244, USA) antibodies for 2 h at room temperature, followed by washing with TBST (TBS containing 0.1% Tween 20) for 5 min five times.

Techniques: Fluorescence, Transfection, Plasmid Preparation, Quantitative RT-PCR, Expressing, Western Blot